Common questions that come up when designing a cryopreservation protocol include what the cooling rate should be and how to ensure the permeating cryoprotecting agent (CPA) has equilibrated inside and outside the cells while minimizing the toxicity of the CPA. Mathematical modeling of the cryopreservation process is a useful tool to investigate the different variables that affect cryopreservation. Understanding the transport of water and the CPA across the cell membrane is critical. This study details a new iterative fitting method that uses non-ideal thermodynamic models to obtain the cell-specific permeability parameters for water and the CPA (Lp and Ps), along with three additional parameters that are required for non-ideal modelling.
Permeability and Osmotic Parameters of Human Umbilical Vein Endothelial Cells and H9C2 Cells under Non-ideal Thermodynamic Assumptions: A Novel Iterative Fitting Method. Gabler Pizarro, L.A. McGann, L.E. and Elliott, J.A.W. The Journal of Physical Chemistry B 2021 125 (47), 12934-12946.